Iron-dextran antibody conjugates: General method for simultaneous staining of two components in high-resolution immunoelectron microscopy.
نویسندگان
چکیده
We report the preparation and properties of an electron-dense antibody conjugate, made by the covalent bonding of an iron-dextran (Imposil) particle to an antibody molecule. Transmission electron microscopic experiments with the Imposil-antibody conjugates demonstrate their suitability as specific immunostains at high resolution, particularly for simultaneous double staining experiments in conjunction with ferritin-antibody conjugates.
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 76 7 شماره
صفحات -
تاریخ انتشار 1979